OP0079 SYNOVIAL FIBROBLASTS – T CELL INTERACTIONS ARE MAJOR DRIVERS OF INFLAMMATION: A CLOSER LOOK INTO THE JOINT
نویسندگان
چکیده
Background Rheumatoid Arthritis (RA) is a chronic inflammatory disease, which characterized by synovial inflammation resulting in bone and cartilage destruction. Crosstalk between activated fibroblast-like synoviocytes (FLS) immune cells, such as CD4 + T within the synovium might amplify joint Objectives To define interaction profile of FLS cells an setting to elucidate its consequence on inflammation. Methods screen for factors that activate RA, isolated were treated with different cytokines transcriptomic changes measured RNA-seq. Fluorescence cell sorting (FACS) purified naïve T-cells from same patients co-cultured cytokine pre-treated FLS. Automated fluorescence microscopy downstream bioinformatic image analysis allowed visualization quantification cell-cell interactions. After co-culture T-cell activation, proliferation differentiation was determined flow cytometry. Results model vivo situation, pre-stimulated pro- anti-inflammatory cytokines. RNA-seq revealed specific activation patterns Correspondingly, we observed distinct – profiles depending used activation. In line profiles, into CD62L high CD45RO memory could be detected. Signatures cytokine-stimulated identified data tissue samples. Conclusion Within this study, describe how induced interactions impact proliferation, differentiation. References [1]Zhang F, Wei K, Slowikowski Fonseka CY, Rao DA, Kelly S, et al. Defining states rheumatoid arthritis tissues integrating single-cell transcriptomics mass Nat Immunol. 2019. [2]Smolen JS, Aletaha D, Barton A, Burmester GR, Emery P, Firestein GS, arthritis. Rev Dis Primers. 2018;4:18001. [3]Smolen McInnes IB. The Lancet. 2016;388(10055):2023-38. [4]Bartok B, GS. Fibroblast-like synoviocytes: key effector Immunol Rev. 2010;233(1):233-55. [5]Zhang Disclosure Interests None declared.
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ژورنال
عنوان ژورنال: Annals of the Rheumatic Diseases
سال: 2022
ISSN: ['1468-2060', '0003-4967']
DOI: https://doi.org/10.1136/annrheumdis-2022-eular.1804